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Revolutionizing Single-Cell RNA-seq With Automated Cell Counters: Insights From Logos Biosystems

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Despite its relative newness, single-cell RNA sequencing (scRNA-seq) has become an essential component of modern biological research.

 

It can be used to characterize abnormal cell populations, discover and analyze rare cell cellular map networks, and discover subtle yet notable heterogeneities.

scRNA-seq has come a long way since its origins in next-generation sequencers from the late 1990s. While traditional sequencing methods measure a bulk of a cell population to determine its “average genome,” scRNA-seq is significantly more precise. It locates and extracts genomes from individual cells, using each cell to tell part of the genetic story of a greater whole.

Although single-cell sequencing provides valuable information, the process has several drawbacks depending on the method used.

For example, Laser Capture Microdissection (LCM) uses a laser to isolate target cells from a complete solid tissue sample located on a microscope slide. This approach is quick, reliable, and also usable on intact tissues, but it requires the user to identify target cells through visual inspection of their morphology. Cells can also be damaged in the process.

Other methods, like Magnetic-Activated Cell Sorting (MACS) or Fluorescence-Activated Cell Sorting (FACS), come with positives and negatives. Regardless of the approach, advancements in single-cell sequencing technology require significant time and investment, making access to newer and more efficient technologies a barrier to progress.

Overcoming Obstacles With Technological Advancement

When manually counting cells in scRNA-seq analysis, adequately going through each cell can take a huge amount of time and resources. This process also has a high margin of error, sometimes making it difficult to justify the effort.

Moreover, when cell counts are inaccurate for scRNA-seq analysis, overall data quality becomes less reliable, making the research outcomes less reliable and further exacerbating the original issues of time and cost.

With so much time and capital going toward this research, the data it produces should be worthwhile. However, the quality of the data ultimately relies on the quality of the sample before processing, which is where Logos Biosystems and their LUNA-FX7 Automated Cell Counter come in.

Enhancing Research Capabilities

Logos Biosystems is a leader in automated cell counting technology and scRNA-seq analysis and is known for developing the award-winning LUNA Cell Counter family.

Founded in 2008, the company has lived up to its motto of “seeing beyond the cell” by working to improve human health through imaging solutions that help researchers gather quality data in a timely fashion.

Their LUNA series of cell counters specializes in improving research accuracy and efficiency by allowing scientists to spend less time dealing with the monotony of cell counting and more time making valuable observations and implementing solutions.

The LUNA-FX7 Automated Cell Counter gives precise and reliable cell counts. It improves the quality of scRNA-seq analysis and takes less time than other automated counters.

This device has many invaluable features, such as increased size for sample throughput, an expanded cell concentration range, built-in QC software, validation slides for fluorescence, and brightfield to allow for daily QC monitoring and reporting.

Making Research Matter

Incorporating an automated cell counter like the LUNA-FX7 more broadly in scRNA-seq would improve research outcomes and accelerate scientific discoveries. Having machines take care of menial tasks frees up time for scientists and researchers to use their critical and creative thinking skills to push progress in their respective fields forward in ways machines couldn’t do alone.

Applications for automated cell counting technology are almost limitless, including research into developmental studies, immunology, oncology, neurobiology, diabetes, microbiology, and much more. Being able to quickly and precisely profile, identify, classify, and discover rare or new cell types from across the human body allows greater insight into these disciplines and what they can do for human health and growth.

The exciting future of single-cell RNA sequencing lies in the seamless integration of these automated technologies. As they become more widely adopted, they will pave the way for more innovative discoveries that could shape the understanding of biology and medicine. With technologies like Logos Biosystems’ LUNA-FX7, the question of whether the scientific community can see transformative discoveries is now mute as it continues to work to enhance precision and efficiency in cell evaluation, which is vital for scRNA-seq experiments to be successful to not only advance science but also improve human health and well-being on a global scale.

 

Rosario is from New York and has worked with leading companies like Microsoft as a copy-writer in the past. Now he spends his time writing for readers of BigtimeDaily.com

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Transform Your Expertise into a Profitable Online Coaching Business with Jon Penberthy

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Transforming your expertise into a successful coaching business requires a strategic approach to monetizing your knowledge, with a strong emphasis on client satisfaction and adaptability to their evolving needs. By prioritizing these factors, you can build a coaching practice that flourishes, provides long-term value to your clients, and supports sustained growth for your business.

The potential for this growth is underscored by the global online coaching market, which was valued at $3.2 billion in 2022 and is projected to reach $11.7 billion by 2032, reflecting a compound annual growth rate (CAGR) of 14% from 2023 to 2032.

Jon Penberthy, founder of AdClients and a leader in online coaching, highlights the significance of the knowledge economy in today’s marketplace. He notes, “The knowledge economy is now worth over half a trillion dollars a year. That means every year, people like you and me are paying others—not for physical products, but for the exchange of knowledge.” This shift presents a compelling opportunity for those willing to leverage their expertise in this evolving market.

Penberthy’s philosophy emphasizes the power of positive thinking and challenges traditional views on credentialism and rigid professional roles. He asserts, “Nowadays you only need to be one step ahead of someone else for them to be willing to hand back some money to learn from you.” His success as a how-to coach exemplifies this approach, showing that with the right mindset, anyone can turn their knowledge into a flourishing business.

Essential Steps to Starting Your Online Business

At the heart of any business plan is the decision about what type of product you will provide in the online marketplace. Jon Penberthy explores various possibilities, from relationship counseling to pet care, ultimately settling on a widely sought-after internet offering: personal health and fitness.

“Let’s say you do not have a personal trainer certificate, but you have figured out a specific nutrition and exercise regime that works,” he states. “There are people out there who want to look how you look and are willing to pay you for your knowledge … the opportunities are endless – you just have to ask yourself what you know that is a little bit more than those around you. That’s the starting point for your own training program.”

The next step involves packaging your training for an online audience, and Penberthy recommends creating a series of recorded videos as an effective approach. This leads to the question of how much to cover in the initial video and the order of presentation.

He suggests finding friends and family who are interested in your topic and willing to learn more. By selecting a few volunteers and teaching them over several weeks for free, while taking diligent notes on what works and what doesn’t, the teaching process will gradually reveal itself.

Build a Sales Funnel

Regardless of how your business attracts customers, potential buyers often follow a similar path, asking common questions and taking comparable steps when deciding whether to make a purchase. A sales funnel is an effective way to visualize this journey, offering valuable insights into the customer experience. It helps you see the sales process through their perspective while also serving as a practical training tool for your sales team.

Penberthy highlights the importance of this approach, “A sales funnel helps transition potential customers from being strangers to ready-to-buy clients,” he explains. By breaking the process into a series of steps, the sales funnel gradually informs and engages potential customers, guiding them toward a purchase decision without overwhelming them with information.

Attracting Attention—The ‘Eyeball’ Factor

Once you’ve understood the initial steps for setting up your online coaching or course, the next challenge is attracting people to your funnel, often referred to as the “eyeball” factor. “Bringing traffic to your site involves content creation and deciding between organic (unpaid) traffic through various social media channels or, if your budget allows, paid traffic,” Penberthy explains.

Penberthy explains that with organic traffic, individuals will be active on social media, creating content designed to build an audience interested in their topic. He adds that if one can invest some funds, paid advertising—especially on YouTube—can be an excellent starting point, as it delivers instant traffic compared to the uncertain outcomes of organic posts.

Once the advertising strategy is established, the next step is to continually refine and enhance the course, making it more concise, message-rich, and easier for potential customers to understand what is being offered.

He emphasizes that this process isn’t just about feeling good about one’s work; it’s about boosting conversions. The more effective the campaign, the more referrals satisfied customers will provide when recommending the program. A stronger program also allows for higher pricing for the services offered.

After refining your online advertising strategies and advancing your course or coaching development, the next step is to scale up. Penberthy suggests that this may initially involve what he refers to as “the pop-up offer” or one-on-one coaching, enabling you to start selling your course in 48 hours or less.

He notes that this phase requires a significant investment of time but is crucial for growing your business with clients who will not only pay for your expertise but also recommend your courses to a broader audience. However, he emphasizes the need to leverage your time effectively, as there are only so many hours in a day.

The key to success in online courses lies in combining “low-ticket” (mass appeal) content with “high-ticket” one-on-one training. “I take the stand-alone low-ticket coaching and wrap it around the one-on-ones to create the concept of “high-ticket” group coaching, which is a limited-subscriber webinar-based training pitched at high-end clients who are willing to pay a premium to overcome their seeming lack of success in the online marketplace,” Penberthy says. 

By implementing this strategy, he adds, you can not only maximize your time in the business space and free up energy for friends and family but also potentially increase your monthly income to four or five figures, ultimately leading to an annual income of six to seven figures.

Jon Penberthy’s insights provide a clear roadmap, emphasizing the importance of understanding your audience, leveraging effective marketing strategies, and continually refining your offerings. By combining low-ticket and high-ticket training approaches, you can maximize your reach while delivering exceptional value to your clients.

As you embark on this path, remember that your knowledge and passion can not only lead to financial success but also empower others to achieve their goals. Embrace the opportunities ahead, and watch as you build a thriving coaching business that makes a lasting impact.

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